Journal: Journal of Zhejiang University-SCIENCE B

Article Title: Anti-CD69 monoclonal antibody treatment inhibits airway inflammation in a mouse model of asthma

doi: 10.1631/jzus.b1400285

Figure Lengend Snippet: Fig. 1 Time line presentation of the OVA protocol and the intraperitoneal (i.p.) administration of DXM and anti-CD69 mAb

Article Snippet: A total of 50 μg hamster anti-mouse CD69 mAb (H1.2F3, R&D Systems, USA) or hamster anti-mouse isotype IgG (R&D Systems, USA) in 100 μl phosphate-buffered solution (PBS) was injected intraperitoneally on Day 26 (6 h after aerosol provocation) and Day 27.

Techniques:

Journal: Journal of Zhejiang University-SCIENCE B

Article Title: Anti-CD69 monoclonal antibody treatment inhibits airway inflammation in a mouse model of asthma

doi: 10.1631/jzus.b1400285

Figure Lengend Snippet: Fig. 5 Effects of anti-CD69 mAb or DXM pretreatment on GM-CSF (a) and IL-5 (b) in BALF of OVA-treated mice Anti-CD69 mAb or DXM pretreatment results in a decrease of IL-5 in BALF of OVA-treated mice. DXM administrated after OVA challenge makes no effect on IL-5. Expression of GM-CSF in BALF was not changed by OVA or anti-CD69 mAb or DXM. The values are presented as mean±SE (n=5–10 mice per group). ** P<0.001, compared with the SAL group, using Student’s t-test

Article Snippet: A total of 50 μg hamster anti-mouse CD69 mAb (H1.2F3, R&D Systems, USA) or hamster anti-mouse isotype IgG (R&D Systems, USA) in 100 μl phosphate-buffered solution (PBS) was injected intraperitoneally on Day 26 (6 h after aerosol provocation) and Day 27.

Techniques: Expressing

Journal: Journal of Zhejiang University-SCIENCE B

Article Title: Anti-CD69 monoclonal antibody treatment inhibits airway inflammation in a mouse model of asthma

doi: 10.1631/jzus.b1400285

Figure Lengend Snippet: Fig. 6 Effect of anti-CD69 mAb on OVA-induced AHR OVA-induced AHR is inhibited by anti-CD69 mAb ad- ministrated after OVA challenge. Airway reactivity (per- cent baseline Penh) in response to increasing doses of nebulized methacholine was assessed by whole-body ple- thysmography (n=10 mice per group). * P<0.05, compared with the SAL group, using Student’s t-test

Article Snippet: A total of 50 μg hamster anti-mouse CD69 mAb (H1.2F3, R&D Systems, USA) or hamster anti-mouse isotype IgG (R&D Systems, USA) in 100 μl phosphate-buffered solution (PBS) was injected intraperitoneally on Day 26 (6 h after aerosol provocation) and Day 27.

Techniques:

Journal: PLoS ONE

Article Title: A Study of T Cell Tolerance to the Tumor-Associated Antigen MDM2: Cytokines Can Restore Antigen Responsiveness, but Not High Avidity T Cell Function

doi: 10.1371/journal.pone.0000353

Figure Lengend Snippet: (A) Lymph nodes cells from 6–8 week old Ag pos and Ag neg mice were stained with antibodies against CD4, CD8 and Vβ7 and analysed by flow cytometry. Numbers in quadrants are percentage of live-gated lymphocytes. (B) Lymph node cells were stained with antibodies against CD8, Vβ7 and the activation markers CD44, CD62L, CD69 and CD25. Histograms display gated viable CD8 + Vβ7 + cells. Numbers in histograms represent the specific MFI of the activation marker staining for the gated population. Data are representative of at least three independent experiments.

Article Snippet: The following mAbs were used for flow cytometric staining: rat-anti-mouse Vβ7 FITC (Serotec, UK), rat-anti-mouse CD4 FITC, rat-anti-mouse CD8α APC, rat-anti-mouse CD8α Cy-Chrome 5 (CY-5), rat-anti-mouse Vβ7 PE, hamster-anti-mouse CD69 PE, rat-anti-mouse CD25 PE, rat-anti-mouse CD62L-FITC, rat-anti-mouse CD44 PE, rat-anti-mouse CD43 activation-associated glycoform-PE (all BD Biosciences).

Techniques: Staining, Flow Cytometry, Activation Assay, Marker